6ZK2

Plant nucleoside hydrolase - ZmNRh2b in complex with forodesine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-19
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9793
Spacegroup name	P 1 21 1
Unit cell lengths	47.920, 148.980, 88.510
Unit cell angles	90.00, 102.40, 90.00

Refinement procedure

Resolution	30.120 - 2.200
R-factor	0.193
Rwork	0.191
R-free	0.22900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4kpo
RMSD bond length	0.010
RMSD bond angle	1.080
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.800	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.163	0.520
Number of reflections	59871	5446
<I/σ(I)>	6.4
Completeness [%]	97.6
Redundancy	3.6
CC(1/2)	0.987	0.827

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	292	PEG 4000, Tris