6ZIO
CRYSTAL STRUCTURE OF NRAS (C118S) IN COMPLEX WITH GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-11 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 37.174, 37.161, 66.245 |
| Unit cell angles | 78.83, 75.24, 64.70 |
Refinement procedure
| Resolution | 19.190 - 1.550 |
| R-factor | 0.239 |
| Rwork | 0.237 |
| R-free | 0.27800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3con |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.950 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.190 | 1.684 |
| High resolution limit [Å] | 1.550 | 1.555 |
| Rmerge | 0.100 | 0.451 |
| Number of reflections | 28360 | 1419 |
| <I/σ(I)> | 7.5 | 2.1 |
| Completeness [%] | 83.5 | |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 278 | 25% PEG 2000, 100mM HEPES PH=7.5 |
