6ZHT

Uba1-Ubc13 disulfide mediated complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-02-18
Detector	DECTRIS PILATUS3 X 2M
Wavelength(s)	0.873
Spacegroup name	C 1 2 1
Unit cell lengths	181.390, 58.270, 137.460
Unit cell angles	90.00, 109.72, 90.00

Refinement procedure

Resolution	24.710 - 2.300
R-factor	0.2091
Rwork	0.206
R-free	0.26320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3cmm
RMSD bond length	0.003
RMSD bond angle	0.682
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.383
High resolution limit [Å]	2.300	2.301
Number of reflections	60597	483
<I/σ(I)>	7.69
Completeness [%]	71.3
Redundancy	2
CC(1/2)	0.997	0.404

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		273	ammonium nitrate, CHES, PEG 4000