6ZG4
Structure of M1-StaR-T4L in complex with HTL0009936 at 2.35A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-11-28 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.03319 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.470, 65.450, 154.830 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.980 - 2.330 |
| R-factor | 0.205 |
| Rwork | 0.204 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2y00 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.970 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.982 | 2.484 |
| High resolution limit [Å] | 2.353 | 2.353 |
| Rpim | 0.194 | 1.525 |
| Number of reflections | 20575 | 674 |
| <I/σ(I)> | 7.1 | |
| Completeness [%] | 89.2 | |
| Redundancy | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | 0.1M NaHEPES pH 7.4-7.8, 0.1M di-Ammonium hydrogenphosphate, 30-38% PEG300 |
