6ZEF
Structure of PP1(7-300) bound to Phactr1 (516-580) at pH 5.25
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-02-07 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97 |
Spacegroup name | P 1 |
Unit cell lengths | 47.510, 57.550, 89.614 |
Unit cell angles | 78.08, 74.67, 81.66 |
Refinement procedure
Resolution | 56.050 - 1.940 |
R-factor | 0.1829 |
Rwork | 0.181 |
R-free | 0.21150 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4mov |
RMSD bond length | 0.005 |
RMSD bond angle | 0.841 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.18_3845) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.050 | 2.010 |
High resolution limit [Å] | 1.940 | 1.940 |
Rmerge | 0.133 | 0.579 |
Rmeas | 0.141 | 0.640 |
Rpim | 0.047 | 0.260 |
Number of reflections | 65136 | 6061 |
<I/σ(I)> | 9.77 | 2.73 |
Completeness [%] | 98.5 | 91.97 |
Redundancy | 8 | |
CC(1/2) | 0.880 | 0.885 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.25 | 293 | 1M LiCl, 0.1 M tri-sodium citrate pH 5.25 and 10 % PEG 6000 |