6ZC4
Small-molecule inhibitors of the PDZ domain of Dishevelled proteins interrupt Wnt signalling
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2009-05-19 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.91841 |
| Spacegroup name | I 4 |
| Unit cell lengths | 76.350, 76.350, 72.570 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.900 - 1.850 |
| R-factor | 0.1874 |
| Rwork | 0.185 |
| R-free | 0.22680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2f0a |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.090 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.900 | 1.900 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmeas | 0.006 | 0.105 |
| Number of reflections | 17796 | 1282 |
| <I/σ(I)> | 20.5 | 2.1 |
| Completeness [%] | 99.8 | |
| Redundancy | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 30% PEG 8000, 0.2 M ammonium sulphate, 0.1 M MES pH 6.5 |
