6ZC4
Small-molecule inhibitors of the PDZ domain of Dishevelled proteins interrupt Wnt signalling
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2009-05-19 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.91841 |
Spacegroup name | I 4 |
Unit cell lengths | 76.350, 76.350, 72.570 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.900 - 1.850 |
R-factor | 0.1874 |
Rwork | 0.185 |
R-free | 0.22680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2f0a |
RMSD bond length | 0.002 |
RMSD bond angle | 1.090 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.900 | 1.900 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmeas | 0.006 | 0.105 |
Number of reflections | 17796 | 1282 |
<I/σ(I)> | 20.5 | 2.1 |
Completeness [%] | 99.8 | |
Redundancy | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 30% PEG 8000, 0.2 M ammonium sulphate, 0.1 M MES pH 6.5 |