6ZAY

Crystal structure of Atg16L in complex with GDP-bound Rab33B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-06-29
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97714
Spacegroup name	P 21 21 21
Unit cell lengths	57.010, 132.110, 154.370
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.572 - 2.400
R-factor	0.1865
Rwork	0.185
R-free	0.21140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1Z06 and 3Q8T
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.572	2.486
High resolution limit [Å]	2.400	2.400
Rmerge	0.117
Number of reflections	46452	4577
<I/σ(I)>	16.22
Completeness [%]	100.0	99.89
Redundancy	13.1	13.7
CC(1/2)		0.971

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.2	277.15	22% PEG3350, 0.2M potassium thiocyanate and 0.1M sodium potassium phosphate