6Z7V
Crystal structure of Mindy2 (C266A) in complex with Lys48 linked di-ubiquitin (K48-Ub2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-11-25 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 94.011, 117.597, 125.884 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.790 - 2.650 |
| R-factor | 0.2148 |
| Rwork | 0.213 |
| R-free | 0.25430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5jkn 1ubq |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.581 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19rc7_4070) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.790 | 47.790 | 2.760 |
| High resolution limit [Å] | 2.650 | 9.550 | 2.650 |
| Rmerge | 0.082 | 0.023 | 0.641 |
| Rmeas | 0.094 | 0.026 | 0.728 |
| Rpim | 0.044 | 0.012 | 0.336 |
| Total number of observations | 176139 | 3599 | 20372 |
| Number of reflections | 40463 | 935 | 4569 |
| <I/σ(I)> | 11.8 | 43.3 | 2.1 |
| Completeness [%] | 98.4 | 95.3 | 99.6 |
| Redundancy | 4.4 | 3.8 | 4.5 |
| CC(1/2) | 0.998 | 0.999 | 0.704 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 50 mM potassium phosphate monobasic, 14% PEG 8000 |
