6Z7B

Variant Surface Glycoprotein VSGsur bound to suramin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-16
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	52.688, 79.219, 118.029
Unit cell angles	90.00, 90.78, 90.00

Refinement procedure

Resolution	48.350 - 1.860
R-factor	0.1961
Rwork	0.190
R-free	0.22200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6z79
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.350	1.926
High resolution limit [Å]	1.860	1.860
Number of reflections	81517	8077
<I/σ(I)>	12.25	2.32
Completeness [%]	99.9	99.77
Redundancy	6.7	6.8
CC(1/2)	0.997	0.725

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	19-24 % PEG 400 100 mM TEA/HCl pH=7.5 10 % (v/v) isopropanol