6Z7A

Variant Surface Glycoprotein VSGsur

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-31
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9184
Spacegroup name	P 21 21 2
Unit cell lengths	47.042, 71.062, 130.453
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.050 - 1.210
R-factor	0.1669
Rwork	0.166
R-free	0.18660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6z79
RMSD bond length	0.019
RMSD bond angle	1.541
Data reduction software	XDS
Data scaling software	pointless
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.050	1.250
High resolution limit [Å]	1.210	1.210
Rmerge	0.096
Rmeas	0.104
Rpim	0.041
Number of reflections	122573	7073
<I/σ(I)>	8.57
Completeness [%]	91.4
Redundancy	6.1
CC(1/2)	0.998	0.174

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	19-24 % PEG 400 100 mM TEA/HCl pH=7.5 10 % (v/v) Isopropanol