6Z3M
Repulsive Guidance Molecule B (RGMB) in complex with Growth Differentiation Factor 5 (GDF5) and Neogenin 1 (NEO1).
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 62 |
| Unit cell lengths | 279.480, 279.480, 142.370 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 69.870 - 5.501 |
| R-factor | 0.331 |
| Rwork | 0.326 |
| R-free | 0.42820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6z3j |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.489 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.16_3549: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.190 | 5.640 |
| High resolution limit [Å] | 5.500 | 5.500 |
| Rmerge | 0.253 | |
| Rpim | 1.103 | |
| Number of reflections | 20764 | 1499 |
| <I/σ(I)> | 5.7 | 0.8 |
| Completeness [%] | 99.8 | |
| Redundancy | 6.3 | |
| CC(1/2) | 0.278 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.1 M NaCl, 20 mM MES pH 6.7, 6.6% w/v PEG 4000 |
