6Z3J

Repulsive Guidance Molecule B (RGMB) in complex with Growth Differentiation Factor 5 (GDF5) (crystal form 1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9762
Spacegroup name	P 1 21 1
Unit cell lengths	36.480, 127.750, 39.910
Unit cell angles	90.00, 99.32, 90.00

Refinement procedure

Resolution	39.380 - 1.650
R-factor	0.1921
Rwork	0.191
R-free	0.21930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6z3h
RMSD bond length	0.005
RMSD bond angle	0.855
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.13rc2_2986: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.380	1.690
High resolution limit [Å]	1.650	1.650
Rmerge	0.088
Number of reflections	43071	3117
<I/σ(I)>	9.5	0.5
Completeness [%]	99.7
Redundancy	6.8
CC(1/2)		0.212

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	294	0.2 M Li2SO4, 0.1 M HEPES pH 7.5, 25% v/v PEG 3350.