6Z3G

Repulsive Guidance Molecule A (RGMA) in complex with Growth Differentiation Factor 5 (GDF5)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 62 2 2
Unit cell lengths	97.650, 97.650, 99.820
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.825 - 2.780
R-factor	0.2365
Rwork	0.234
R-free	0.28780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4uhy
RMSD bond length	0.005
RMSD bond angle	0.624
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.13rc2_2986: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.910	2.850
High resolution limit [Å]	2.780	2.780
Rmerge	0.090	4.129
Number of reflections	7375	494
<I/σ(I)>	23.4	0.8
Completeness [%]	97.8
Redundancy	18.5
CC(1/2)		0.592

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	0.2 M ammonium acetate, 0.1 M sodium citrate tribasic dihydrate pH 5.5, 24% v/v polyethylene glycol (PEG) 400.