6Z1N

Structure of the human heterotetrameric cis-prenyltransferase complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-24
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.976
Spacegroup name	H 3 2
Unit cell lengths	184.074, 184.074, 112.593
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.983 - 2.300
R-factor	0.1905
Rwork	0.189
R-free	0.22290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1x09
RMSD bond length	0.005
RMSD bond angle	0.701
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MrBUMP
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.019	2.440
High resolution limit [Å]	2.300	2.300
Rmeas	0.170	1.978
Number of reflections	32476	5182
<I/σ(I)>	16.32	1.56
Completeness [%]	100.0	100
Redundancy	21.1
CC(1/2)	0.999	0.677

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	0.1 M NaCl, 0.1 M NaP pH 7.0, 33% w/v PEG 300, 760 mM FPP