6YYH

Crystal structure of beta-D-xylosidase from Dictyoglomus thermophilum in ligand-free form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.99
Spacegroup name	P 31 2 1
Unit cell lengths	159.140, 159.140, 100.150
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.060 - 2.720
R-factor	0.2024
Rwork	0.200
R-free	0.25630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1px8
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.17.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.060	47.060	2.880
High resolution limit [Å]	2.720	8.040	2.720
Rmerge	0.107	0.031	1.207
Rmeas	0.110	0.032	1.237
Total number of observations	827425
Number of reflections	39616	1640	6263
<I/σ(I)>	23.69	85.07	2.74
Completeness [%]	99.8	99.5	98.8
Redundancy	20.886	19.111	21.074
CC(1/2)	1.000	1.000	0.878

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	293	0.1 M Ammonium Sulfate 0.1 M Na citrate pH 5.6 10% PEG4000