6YXZ
Structure and activity of the GH20 beta-N-beta-N-acetylhexosaminidase from Bifidobacterium bifidum
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-05-18 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.566, 126.750, 152.710 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 63.380 - 1.750 |
Rwork | 0.160 |
R-free | 0.21970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | generated by MrBUMP |
RMSD bond length | 0.009 |
RMSD bond angle | 1.511 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | SHELXDE |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 63.380 | 1.780 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 1.654 | |
Rpim | 0.969 | |
Number of reflections | 111519 | 5474 |
<I/σ(I)> | 9 | 1.1 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.4 | 7.4 |
CC(1/2) | 0.997 | 0.610 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 21.6 mg/mL of purified protein in the buffer of tris 25 mM pH 8.0 and NaCl 200 mM is mixed with PEG 3350 23%, 0.1 M bis-tris propane PH 6.5 and 0.2 M NaBr at 1:1 ratio. |