6YXQ

Crystal structure of a DNA repair complex ASCC3-ASCC2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-20
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97957
Spacegroup name	I 4
Unit cell lengths	158.108, 158.108, 69.365
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.960 - 2.700
R-factor	0.2063
Rwork	0.204
R-free	0.24740
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.907
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	AutoSol
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.964	2.860
High resolution limit [Å]	2.700	2.700
Rmerge	0.164
Number of reflections	45845	7271
<I/σ(I)>	5.89
Completeness [%]	99.0
Redundancy	3.5
CC(1/2)	0.995	0.139

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	MES-NaOH, pH 6.5, polyethylene glycol 3350, 2-methyl-2,4-pentanediol and methanol