6YXF
Cryogenic human adiponectin receptor 2 (ADIPOR2) with Gd-DO3 ligand determined by Serial Crystallography (SSX) using CrystalDirect
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-22 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.72 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 74.640, 100.650, 111.200 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.670 - 3.025 |
R-factor | 0.2163 |
Rwork | 0.214 |
R-free | 0.25930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5lwy |
RMSD bond length | 0.010 |
RMSD bond angle | 1.150 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3 (29-NOV-2019)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.100 |
High resolution limit [Å] | 3.000 | 3.010 |
Number of reflections | 31468 | 2336 |
<I/σ(I)> | 5.06 | |
Completeness [%] | 100.0 | |
Redundancy | 14.98 | |
CC(1/2) | 0.980 | 0.300 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 293 | 30 or 50 nl bolus overlaid with 600 nl precipitant solution using either a Mosquito LCP robot (SPT Labtech) in CrystalDirect plate-2. The crystallisation condition is made of 42.3% PEG 400, 110 mM potassium citrate, 100 mM HEPES pH 7.0. 60-120 nL of Gd-DO3 soaking solution with a final conc. of 10 - 25 mM was delivered onto LCP crystallisation drop. Crystallisation experiments were carried out at the HTX facility of EMBL Grenoble. LCP bolus were harvested automatically at cryogenic condition using CrystalDirect technology. |