6YX5

Structure of DrrA from Legionella pneumophilia in complex with human Rab8a

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-07
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.966
Spacegroup name	P 3 2 1
Unit cell lengths	142.309, 142.309, 76.616
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.060 - 2.140
R-factor	0.2084
Rwork	0.207
R-free	0.23850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3nku 4lhv
RMSD bond length	0.004
RMSD bond angle	1.256
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0253)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.060	2.220
High resolution limit [Å]	2.140	2.140
Rmerge	0.200	2.430
Rmeas	0.206	2.500
Rpim	0.045	0.570
Number of reflections	49091	4745
<I/σ(I)>	15.57	0.93
Completeness [%]	99.7	96.97
Redundancy	20	18.3
CC(1/2)	0.990	0.430

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	293	0.1M bicine, pH 9, 2.4M ammonium sulphate