6YTI
CLK1 bound with ETH1610 (Cpd 17)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-23 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 91.518, 63.873, 79.410 |
| Unit cell angles | 90.00, 118.83, 90.00 |
Refinement procedure
| Resolution | 48.030 - 2.400 |
| R-factor | 0.1982 |
| Rwork | 0.195 |
| R-free | 0.25550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1z57 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.689 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.030 | 47.980 | 2.490 |
| High resolution limit [Å] | 2.400 | 8.980 | 2.400 |
| Rmerge | 0.153 | 0.034 | 1.064 |
| Rmeas | 0.166 | 0.037 | 1.149 |
| Rpim | 0.062 | 0.014 | 0.433 |
| Total number of observations | 2249 | 11317 | |
| Number of reflections | 15727 | 325 | 1633 |
| <I/σ(I)> | 10.1 | 39.4 | 1.9 |
| Completeness [%] | 99.4 | 99.3 | 98.9 |
| Redundancy | 7 | 6.9 | 6.9 |
| CC(1/2) | 0.996 | 0.999 | 0.707 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.3 | 277 | 29% PEG 6k, 0.1M bicine |
