6YT7
GLYCOSYLATED KNOB/DUMMY-HOLE FC FRAGMENT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-13 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.015, 75.103, 149.445 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 67.110 - 1.550 |
| R-factor | 0.236 |
| Rwork | 0.234 |
| R-free | 0.27310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5hy9 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_3893) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.830 | 1.690 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rpim | 0.020 | |
| Number of reflections | 64377 | 3224 |
| <I/σ(I)> | 23.1 | 1.6 |
| Completeness [%] | 95.4 | 61.8 |
| Redundancy | 6.6 | 6.9 |
| CC(1/2) | 1.000 | 0.700 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 25% (w/v) PEG2000 MME, 0.1M HEPES |
