6YRB

Crystal structure of the tetramerization domain of the glycoprotein Gn (Andes virus) at pH 7.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.8
Spacegroup name	I 4
Unit cell lengths	67.854, 67.854, 121.891
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.699 - 2.351
R-factor	0.228
Rwork	0.226
R-free	0.26550
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	SHELXDE
Refinement software	PHENIX (1.14rc3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	37.700	37.700	2.430
High resolution limit [Å]	2.350	9.100	2.350
Rmerge	0.199	0.141	1.294
Rmeas	0.209	0.149	1.366
Rpim	0.066	0.048	0.435
Total number of observations	114803	2055	10501
Number of reflections	11445	210	1080
<I/σ(I)>	8.1	15.7	1.7
Completeness [%]	99.6	99	96.4
Redundancy	10	9.8	9.7
CC(1/2)	0.988	0.986	0.455

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.2M NaCl, 0.1M Hepes 7.5, 35% MPD