6YPT
X-ray structure of Turnip Yellow Mosaic Virus PRO/DUB in complex with Ubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-21 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.930, 51.860, 125.250 |
| Unit cell angles | 90.00, 98.37, 90.00 |
Refinement procedure
| Resolution | 37.460 - 3.663 |
| R-factor | 0.2094 |
| Rwork | 0.206 |
| R-free | 0.28270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5lw5 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.495 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.460 | 3.810 |
| High resolution limit [Å] | 3.660 | 3.663 |
| Rmerge | 0.215 | |
| Number of reflections | 5303 | 715 |
| <I/σ(I)> | 4.14 | |
| Completeness [%] | 97.0 | 83.4 |
| Redundancy | 3.3 | 3.3 |
| CC(1/2) | 0.985 | 0.415 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 21% PEG-20K + 0.1M MES-NaOH pH6.5 |
