6YPR

Human histidine triad nucleotide-binding protein 2 (hHINT2) refined to 1.26 A in H32 space group

This is a non-PDB format compatible entry.

Replaces:  4NJY

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-03
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.0332
Spacegroup name	H 3 2
Unit cell lengths	72.076, 72.076, 103.576
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.043 - 1.260
Rwork	0.117
R-free	0.14270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3tw2
RMSD bond length	0.018
RMSD bond angle	2.077
Data reduction software	XDS
Data scaling software	Aimless (0.5.27)
Phasing software	MOLREP (11.4.06)
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.043	1.280
High resolution limit [Å]	1.260	1.260
Rmerge	0.058	0.645
Rmeas	0.073	0.815
Rpim	0.043	0.492
Number of reflections	28102	1352
<I/σ(I)>	12.6	2
Completeness [%]	99.8	99.8
Redundancy	4.8	4.5
CC(1/2)	0.998	0.685

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	281	0.8 M Na/K Tartrate, 0.1 M HEPES 7.5