6YOO

Structure of SAMM50 LIR bound to GABARAPL1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-30
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97
Spacegroup name	P 21 21 21
Unit cell lengths	43.980, 54.040, 62.070
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.760 - 1.060
R-factor	0.1349
Rwork	0.134
R-free	0.14750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6hoi
RMSD bond length	0.010
RMSD bond angle	1.081
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.760	1.100
High resolution limit [Å]	1.060	1.060
Rmerge	0.060	2.500
Rpim	0.010	0.790
Number of reflections	825136	72954
<I/σ(I)>	17.2	1.2
Completeness [%]	99.4
Redundancy	12.2
CC(1/2)	1.000	0.620

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	24% w/v PEG 6000, 10 mM ZnCl2 and 0.1 M Tris pH 7.5