6YNP

Crystal structure of YTHDC1 with compound T96C1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-05
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	39.620, 103.290, 42.300
Unit cell angles	90.00, 105.49, 90.00

Refinement procedure

Resolution	40.760 - 1.100
R-factor	0.1917
Rwork	0.191
R-free	0.19760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4r3h
RMSD bond length	0.005
RMSD bond angle	0.811
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.800	1.170
High resolution limit [Å]	1.100	1.100
Number of reflections	131690	20992
<I/σ(I)>	15.08
Completeness [%]	99.5
Redundancy	4.29
CC(1/2)	1.000	0.613

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	25% PEG3350, 0.2M Ammonium Sulphate, 0.1M bis-tris, pH 6.5