6YN0
Structure of E. coli PBP1b with a FtsN peptide activating transglycosylase activity
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-14 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.978565 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 63.143, 283.021, 62.693 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.170 - 2.400 |
| R-factor | 0.226 |
| Rwork | 0.225 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5hla |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.920 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.170 | 2.510 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.250 | |
| Rmeas | 0.265 | 3.810 |
| Number of reflections | 25830 | 6891 |
| <I/σ(I)> | 8.1 | 1.5 |
| Completeness [%] | 95.5 | 85.9 |
| Redundancy | 8.5 | 7.2 |
| CC(1/2) | 0.995 | 0.279 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | (NH4)2SO4 0.1M, sodium Formate 0.3M, Tris-Hcl 0,1M, low molecular weight poly-glutamic acids (PGA-LM) 3% w/v, PEG 550 MME 20% v/v |
