6YMD
Crystal structure of serine hydroxymethyltransferase from Aphanothece halophytica in the covalent complex with malonate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-08-10 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.050, 94.202, 142.972 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 71.490 - 1.250 |
| R-factor | 0.1289 |
| Rwork | 0.129 |
| R-free | 0.15010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kkj |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.203 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.490 | 1.320 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmeas | 0.053 | 0.057 |
| Number of reflections | 226857 | 32794 |
| <I/σ(I)> | 21.4 | 1.8 |
| Completeness [%] | 97.1 | 87.5 |
| Redundancy | 7.9 | 6.8 |
| CC(1/2) | 1.000 | 0.730 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 292 | 0.2 M sodium malonate at pH 5.0 and 20% polyethylene glycol 3350 |
