6YL2
Structural and DNA binding studies of the transcriptional repressor Rv2506 (BkaR) from Mycobacterium tuberculosis supports a role in L-Leucine catabolism
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-03-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97242 |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 58.409, 66.000, 179.732 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 62.030 - 3.150 |
| R-factor | 0.2679 |
| Rwork | 0.267 |
| R-free | 0.28440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6yj2 |
| RMSD bond length | 0.027 |
| RMSD bond angle | 1.859 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.030 | 3.200 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rmerge | 0.038 | 0.285 |
| Rpim | 0.020 | 0.181 |
| Number of reflections | 12331 | 597 |
| <I/σ(I)> | 11.7 | 1.8 |
| Completeness [%] | 98.7 | 99.5 |
| Redundancy | 4.3 | 4.3 |
| CC(1/2) | 0.999 | 0.982 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 289 | 0.2 M sodium acetate trihydrate, 0.1 M sodium citrate pH 5.5, 5% (w/v) PEG 4000. |
