6YL2

Structural and DNA binding studies of the transcriptional repressor Rv2506 (BkaR) from Mycobacterium tuberculosis supports a role in L-Leucine catabolism

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-03-06
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97242
Spacegroup name	P 2 2 21
Unit cell lengths	58.409, 66.000, 179.732
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.030 - 3.150
R-factor	0.2679
Rwork	0.267
R-free	0.28440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6yj2
RMSD bond length	0.027
RMSD bond angle	1.859
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.030	3.200
High resolution limit [Å]	3.150	3.150
Rmerge	0.038	0.285
Rpim	0.020	0.181
Number of reflections	12331	597
<I/σ(I)>	11.7	1.8
Completeness [%]	98.7	99.5
Redundancy	4.3	4.3
CC(1/2)	0.999	0.982

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	289	0.2 M sodium acetate trihydrate, 0.1 M sodium citrate pH 5.5, 5% (w/v) PEG 4000.