6YK6

Structure of the AMPA receptor GluA2o ligand-binding domain (S1S2J) in complex with the compound (S)-1-(2'-Amino-2'-carboxyethyl)furo[3,4-d]pyrimidin-2,4-dione at resolution 1.47A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-3
Synchrotron site	MAX II
Beamline	I911-3
Temperature [K]	105
Detector technology	CCD
Collection date	2013-09-10
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.00001
Spacegroup name	P 21 21 2
Unit cell lengths	59.280, 95.071, 48.408
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.535 - 1.469
R-factor	0.1524
Rwork	0.151
R-free	0.17150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1syh
Data reduction software	XDS
Data scaling software	SCALA (3.3.20)
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.535	47.535	1.550
High resolution limit [Å]	1.469	4.640	1.470
Rmerge		0.021	0.375
Rmeas	0.047	0.024	0.422
Rpim	0.021	0.011	0.190
Total number of observations	236886	7755	31519
Number of reflections	47393	1654	6735
<I/σ(I)>	20.7	53.9	3.3
Completeness [%]	99.8	99.3	98.7
Redundancy	5	4.7	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	279	24.4% PEG4000, 0.15M lithium sulfate, 0.1M phosphate-citrate