6YK1
Crystal structure of mouse pyridoxal kinase in complex with ATP-gamma-S and artesunate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9724 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 279.380, 53.037, 110.149 |
| Unit cell angles | 90.00, 91.64, 90.00 |
Refinement procedure
| Resolution | 47.200 - 2.400 |
| R-factor | 0.2113 |
| Rwork | 0.209 |
| R-free | 0.25380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yxt |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.550 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.17.1_3660: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.200 | 2.460 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.046 | 1.069 |
| Rpim | 0.053 | 0.704 |
| Number of reflections | 63594 | 6235 |
| <I/σ(I)> | 8.1 | 1.1 |
| Completeness [%] | 1.0 | 99.6 |
| Redundancy | 3.4 | 3.2 |
| CC(1/2) | 0.996 | 0.541 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.18-0.24 M sodium thiocyanate and 18-26% PEG 3350 |
