6YK0
Crystal structure of mouse pyridoxal kinase in complex with ATP-gamma-S
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9724 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 278.600, 53.021, 109.851 |
| Unit cell angles | 90.00, 91.75, 90.00 |
Refinement procedure
| Resolution | 47.160 - 2.900 |
| R-factor | 0.2347 |
| Rwork | 0.234 |
| R-free | 0.25490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yxt |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.731 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.17.1_3660: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.160 | 3.030 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.100 | 0.739 |
| Rmeas | 0.083 | 0.631 |
| Rpim | 0.068 | 0.488 |
| Number of reflections | 36069 | 3532 |
| <I/σ(I)> | 9.3 | 1.6 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 3.4 | 3.5 |
| CC(1/2) | 0.995 | 0.600 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.18-0.24 M sodium thiocyanate and 18-26% PEG 3350 |
