6YJX
Structure of Hen egg-white lysozyme crystallized with PAS polypeptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-08-30 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.9184 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 79.468, 79.468, 37.848 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.110 - 1.200 |
R-factor | 0.1472 |
Rwork | 0.146 |
R-free | 0.17540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5t3f |
RMSD bond length | 0.012 |
RMSD bond angle | 1.889 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 28.110 | 28.110 | 1.300 |
High resolution limit [Å] | 1.200 | 10.000 | 1.200 |
Rmerge | 0.037 | 0.025 | 0.911 |
Rmeas | 0.040 | 0.027 | 0.973 |
Total number of observations | 320914 | ||
Number of reflections | 38399 | 80 | 8070 |
<I/σ(I)> | 26.02 | 66.32 | 2.44 |
Completeness [%] | 99.8 | 88.9 | 99.8 |
Redundancy | 8.357 | 5.7 | 8.178 |
CC(1/2) | 1.000 | 1.000 | 0.853 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 12.5 % (w/v) PA200 0.1 M NaOAc 0.2 M NaCl |