6YHU

Crystal structure of the nsp7-nsp8 complex of SARS-CoV-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-25
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.541870
Spacegroup name	P 1 21 1
Unit cell lengths	56.420, 49.660, 64.750
Unit cell angles	90.00, 106.41, 90.00

Refinement procedure

Resolution	38.787 - 2.000
R-factor	0.215531271303
Rwork	0.214
R-free	0.23934
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ahm
RMSD bond length	0.002
RMSD bond angle	0.603
Data reduction software	XDS (Mar 15, 2019)
Data scaling software	XDS (Mar 15, 2019)
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.790	2.071
High resolution limit [Å]	2.000	2.000
Rmerge	0.074	0.522
Rmeas	0.081
Number of reflections	23341	2237
<I/σ(I)>	17.1	2.88
Completeness [%]	99.4
Redundancy	6.4
CC(1/2)	0.999	0.902

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2 M Magnesium chlorid hexahydrate, 0.1 M Tris pH 8.5, 30% PEG 4000