6YHU

Crystal structure of the nsp7-nsp8 complex of SARS-CoV-2

Summary for 6YHU

• Entry DOI: 10.2210/pdb6yhu/pdb
• Descriptor: Replicase polyprotein 1a (3 entities in total)
• Functional Keywords: nsp7, nsp8, sars-cov-2, ncov-2019, covid-19, viral protein
• Biological source: Severe acute respiratory syndrome coronavirus 2 (2019-nCoV); More
• Total number of polymer chains: 4
• Total formula weight: 41636.16

Authors

Konkolova, E.,Klima, M.,Boura, E. (deposition date: 2020-03-31, release date: 2020-04-29, Last modification date: 2024-11-06)

Primary citation

Konkolova, E.,Klima, M.,Nencka, R.,Boura, E.
Structural analysis of the putative SARS-CoV-2 primase complex.
J.Struct.Biol., 211:107548-107548, 2020

PubMed Abstract: We report the crystal structure of the SARS-CoV-2 putative primase composed of the nsp7 and nsp8 proteins. We observed a dimer of dimers (2:2 nsp7-nsp8) in the crystallographic asymmetric unit. The structure revealed a fold with a helical core of the heterotetramer formed by both nsp7 and nsp8 that is flanked with two symmetry-related nsp8 β-sheet subdomains. It was also revealed that two hydrophobic interfaces one of approx. 1340 Å connects the nsp7 to nsp8 and a second one of approx. 950 Å connects the dimers and form the observed heterotetramer. Interestingly, analysis of the surface electrostatic potential revealed a putative RNA binding site that is formed only within the heterotetramer.

PubMed: 32535228
DOI: 10.1016/j.jsb.2020.107548

Experimental method

X-RAY DIFFRACTION (2 Å)