6YGD

Crystal structure of the NatC complex bound to Gag peptide and CoA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	P 21 21 21
Unit cell lengths	48.136, 134.927, 165.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.337 - 2.752
R-factor	0.2061
Rwork	0.204
R-free	0.24800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6yga
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.8.1)
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.337	45.337	2.920
High resolution limit [Å]	2.750	8.140	2.750
Rmerge	0.194	0.034	1.342
Rmeas	0.211	0.037	1.456
Total number of observations	188898
Number of reflections	28788	1235	4458
<I/σ(I)>	9.33	42.02	1.32
Completeness [%]	99.5	99	97.5
Redundancy	6.562	5.678	6.683
CC(1/2)	0.996	0.999	0.598

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.3	293	14.5% PEG 4000, 150 mM ammonium iodide and 100 mM sodium citrate, pH 6.3