6YEH
Arabidopsis thaliana glutamate dehydrogenase isoform 1 in apo form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-04-06 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.8266 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 93.570, 99.169, 318.084 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 89.770 - 2.590 |
R-factor | 0.1835 |
Rwork | 0.183 |
R-free | 0.23460 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | AtGDH1-NAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.849 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 90.000 | 88.900 | 2.760 |
High resolution limit [Å] | 2.590 | 7.950 | 2.590 |
Rmerge | 0.190 | 0.043 | 1.070 |
Rpim | 0.074 | 0.025 | 0.390 |
Number of reflections | 69315 | 3467 | 3405 |
<I/σ(I)> | 9.6 | 26.4 | 2.3 |
Completeness [%] | 88.9 | 99.3 | 42.2 |
Redundancy | 7.4 | 6.3 | 8.5 |
CC(1/2) | 0.995 | 0.998 | 0.581 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 292 | 20% w/v PEG 4000, 100 mM Tris-HCl pH 7.5, 33% (v/v) MPD |