6YD6

SaFtsZ-UCM152 (comp.20)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97918
Spacegroup name	C 1 2 1
Unit cell lengths	71.744, 50.184, 88.374
Unit cell angles	90.00, 111.06, 90.00

Refinement procedure

Resolution	40.160 - 1.700
R-factor	0.1667
Rwork	0.165
R-free	0.20040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3v0a
RMSD bond length	0.010
RMSD bond angle	1.141
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.160	1.770
High resolution limit [Å]	1.700	1.700
Rmerge	0.030	0.300
Rmeas	0.040	0.430
Rpim	0.030	0.300
Number of reflections	31638	3192
<I/σ(I)>	9.8	2.03
Completeness [%]	97.4
Redundancy	3
CC(1/2)	0.990	0.820

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	25% PEG5K MME, 10% EthGly, 0.2 M Li2SO4, 0.1 M Tris pH 8.5