6YCQ

Crystal structure of the DNA binding domain of Arabidopsis thaliana Auxin Response Factor 1 (AtARF1) in complex with High Affinity DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-03-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9793
Spacegroup name	P 1 21 1
Unit cell lengths	43.304, 102.784, 127.039
Unit cell angles	90.00, 98.04, 90.00

Refinement procedure

Resolution	47.570 - 1.650
R-factor	0.173
Rwork	0.172
R-free	0.19910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ldx
RMSD bond length	0.008
RMSD bond angle	0.963
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.580	1.680
High resolution limit [Å]	1.650	1.651
Number of reflections	75146	82
<I/σ(I)>	15.9
Completeness [%]	88.1
Redundancy	3.4
CC(1/2)	0.999	0.512

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	B10 condition of the Morpheus HT screen: 0.09 M (0.3M Sodium fluoride, 0.3M Sodium bromide, 0.3M Sodium iodide); 0.1 M (Tris (base), BICINE pH8.5); 20% v/v Ethylene glycol; 10 % w/v PEG 8000