6YAX

Crystal structure of CD32b (Fc Gamma Receptor IIb) in complex with Human IgG1 Fab fragment (5C05)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-24
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.97
Spacegroup name	P 43
Unit cell lengths	95.728, 95.728, 186.038
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.860 - 2.800
Rwork	0.230
R-free	0.26440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5occ
RMSD bond length	0.004
RMSD bond angle	1.383
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.860	2.870
High resolution limit [Å]	2.800	2.800
Number of reflections	41024	3003
<I/σ(I)>	9.1
Completeness [%]	99.3
Redundancy	7.2
CC(1/2)	0.995	0.491

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.2 M 1,6-hexanediol, 0.2 M 1-butanol, 0.2 M 1,2-propanediol, 0.2 M 2- propanol, 0.2 M 1,4-butanediol, 0.2 M 1,3-propanediol, 1.0 M tris (base), BICINE, 40% v/v PEG 500 MME, 20% w/v PEG 20000