6Y7N
The crystal structure of the eight-bladed symmetrical designer protein Tako8 in the presence of tellurotungstic Anderson-Evans (TEW)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-01 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97951 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 73.586, 60.929, 73.613 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.930 - 1.600 |
R-factor | 0.2188 |
Rwork | 0.217 |
R-free | 0.24950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6g6m |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.16) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.940 | 1.630 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.025 | 0.074 |
Rmeas | 0.033 | 0.103 |
Rpim | 0.023 | 0.070 |
Number of reflections | 85806 | 4215 |
<I/σ(I)> | 21.4 | 8.3 |
Completeness [%] | 99.8 | |
Redundancy | 3.3 | |
CC(1/2) | 0.997 | 0.994 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 4.5 | 293 | 0.1M sodium acetate, 3.1M sodium chloride |