6Y58

Binary complex of 14-3-3 sigma (C38N) with the Estrogen Related Receptor gamma (LBD) phosphopeptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	RIGAKU MICROMAX-003
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-01-04
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.54
Spacegroup name	C 2 2 21
Unit cell lengths	82.297, 111.690, 62.593
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.127 - 1.904
R-factor	0.1762
Rwork	0.174
R-free	0.20880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3p1n
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.430	1.940
High resolution limit [Å]	1.900	1.900
Number of reflections	22781	1659
<I/σ(I)>	8.6	3
Completeness [%]	99.3
Redundancy	4
CC(1/2)	0.992	0.873

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.095 M HEPES (pH 7.1), 0.19 M CaCl2, 27% (v/v) PEG 400 and 5% (v/v) glycerol