6Y4O

Calmodulin bound to cardiac ryanodine receptor (RyR2) calmodulin binding domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-02-19
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	38.037, 43.557, 90.102
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.303 - 1.835
R-factor	0.179726606149
Rwork	0.177
R-free	0.20757
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bcx
RMSD bond length	0.009
RMSD bond angle	0.905
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.303	1.870
High resolution limit [Å]	1.835	1.840
Number of reflections	13605	765
<I/σ(I)>	21.7
Completeness [%]	99.2
Redundancy	6.2
CC(1/2)	1.000	0.854

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298.15	0.1 M Sodium Acetate pH 4.70 and 23 % PEG 550 MME