6Y4M
Structure of Tubulin Tyrosine Ligase in Complex with Tb111
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-06 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 103.961, 152.590, 185.989 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.900 - 3.340 |
R-factor | 0.2085 |
Rwork | 0.207 |
R-free | 0.23410 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.002 |
RMSD bond angle | 1.194 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.520 |
High resolution limit [Å] | 3.340 | 3.340 |
Rmerge | 0.216 | 0.683 |
Rmeas | 0.245 | 0.772 |
Rpim | 0.114 | 0.353 |
Number of reflections | 43404 | 6256 |
<I/σ(I)> | 5.1 | |
Completeness [%] | 99.5 | |
Redundancy | 4.2 | |
CC(1/2) | 0.975 | 0.617 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.03 M calcium chloride, 0.03 M magnesium chloride, 0.10 M MES pH 6.50, 3.20% w/v PEG 4000, 5.00% w/v glycerol |