6Y4G

Crystal structure of the human METTL3-METTL14 complex bound to Sinefungin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-16
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 32 2 1
Unit cell lengths	64.030, 64.030, 225.780
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.640 - 1.900
R-factor	0.1851
Rwork	0.183
R-free	0.21750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5l6d
RMSD bond length	0.007
RMSD bond angle	0.891
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.156	2.020
High resolution limit [Å]	1.900	1.900
Number of reflections	43464	6909
<I/σ(I)>	17.96
Completeness [%]	99.9
Redundancy	9.83
CC(1/2)	0.999	0.766

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	20% PEG 3350, 400mM Mg acetate