6Y30
NG domain of human SRP54 T115A mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-06-04 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.972 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 85.795, 53.903, 91.366 |
Unit cell angles | 90.00, 105.97, 90.00 |
Refinement procedure
Resolution | 53.257 - 2.650 |
R-factor | 0.278 |
Rwork | 0.276 |
R-free | 0.30860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6y2z |
RMSD bond length | 0.002 |
RMSD bond angle | 0.459 |
Data reduction software | Aimless |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 53.260 | 2.750 |
High resolution limit [Å] | 2.650 | 2.650 |
Rpim | 0.120 | 0.590 |
Number of reflections | 22749 | 2164 |
<I/σ(I)> | 7.51 | |
Completeness [%] | 95.9 | |
Redundancy | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 1M Potassium Citrate |