6XX0
Crystal structure of NEMO in complex with Ubv-LIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2020-01-25 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 70.323, 80.773, 84.929 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.420 - 2.600 |
| R-factor | 0.2437 |
| Rwork | 0.238 |
| R-free | 0.29060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3f89 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.309 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.990 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 101221 | 2213 |
| <I/σ(I)> | 10.7 | |
| Completeness [%] | 100.0 | |
| Redundancy | 6.6 | |
| CC(1/2) | 0.997 | 0.548 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 22.5% polyethylene glycol 3350, 0.1M Magnesium chloride, 0.1M Try-HCl, pH 7.0 |
