6XW5

Crystal structure of murine norovirus P domain in complex with Nanobody NB-5820

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.072270
Spacegroup name	P 41 21 2
Unit cell lengths	101.720, 101.720, 228.520
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.860 - 1.720
R-factor	0.1629
Rwork	0.161
R-free	0.19110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lq6
RMSD bond length	0.012
RMSD bond angle	1.735
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.860	49.860	1.760
High resolution limit [Å]	1.720	7.680	1.720
Rmerge	0.066	0.026	0.791
Rmeas	0.069	0.027	0.824
Total number of observations	1664812
Number of reflections	127876	1643	9247
<I/σ(I)>	22.34	62.72	3.04
Completeness [%]	99.9	99.5	99
Redundancy	13.019	12.247	12.457
CC(1/2)	1.000	1.000	0.935

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	25% PEG3000, 0.1M Tris pH 8.5