6XVD

Crystal structure of complex of urokinase and a upain-1 variant(W3F) in pH7.4 condition

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	H 3
Unit cell lengths	121.292, 121.292, 43.000
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.830 - 1.400
R-factor	0.1871
Rwork	0.186
R-free	0.20250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4dva
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (v1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.650	1.450
High resolution limit [Å]	1.400	1.400
Rmerge	0.056	0.056
Number of reflections	46337	46337
<I/σ(I)>	25.96
Completeness [%]	99.9
Redundancy	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	293	0.05 M sodium citrate at pH 4.5, 1.95 M (NH4)2SO4, 0.05% NaN3, and 5% PEG400