6XUS
RNA dodecamer with a 6-hydrazino-2-aminopurine modified base
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 41.120, 35.140, 31.870 |
| Unit cell angles | 90.00, 129.43, 90.00 |
Refinement procedure
| Resolution | 24.620 - 1.100 |
| R-factor | 0.1542 |
| Rwork | 0.153 |
| R-free | 0.17370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2q1r |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.808 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.070 |
| High resolution limit [Å] | 1.040 | 1.040 |
| Rmeas | 0.050 | 261.000 |
| Number of reflections | 106381 | 1200 |
| <I/σ(I)> | 13.32 | 0.29 |
| Completeness [%] | 99.1 | 98.6 |
| Redundancy | 6.3 | |
| CC(1/2) | 1.000 | 0.148 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | MPD, Na Cacodylate, spermine, NaCl, MgCl2 |
